CHD1 Antibody, KO Validated

ProSci
Product Code: PSI-23-252
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-252-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using CHD1 Antibody (23-252) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (23-252) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 5
Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CHD1 Antibody (23-252) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (23-252) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (23-252) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CHD1, chromodomain helicase DNA binding protein 1, DKFZp686E2337,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
NCBI Gene ID #:
1105
NCBI Official Name:
chromodomain helicase DNA binding protein 1
NCBI Official Symbol:
CHD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 197kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O14646
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.