EEF1G Antibody

ProSci
Product Code: PSI-23-259
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-259-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EEF1G antibody (23-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using EEF1G antibody (23-259) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human esophagus using EEF1G antibody (23-259) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of C6 cells using EEF1G Polyclonal Antibody (23-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using EEF1G Polyclonal Antibody (23-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EEF1G antibody (23-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using EEF1G antibody (23-259) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EEF1G antibody (23-259) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EEF1G Polyclonal Antibody (23-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using EEF1G Polyclonal Antibody (23-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EEF1G, EF1G, GIG35
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1).
NCBI Gene ID #:
1937
NCBI Official Name:
eukaryotic translation elongation factor 1 gamma
NCBI Official Symbol:
EEF1G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P26641
User NOte:
Optimal dilutions for each application to be determined by the researcher.