EYA3 Antibody

ProSci
Product Code: PSI-23-260
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-260-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EYA3 antibody (23-260) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using EYA3 antibody (23-260) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody (23-260) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
EYA3,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
NCBI Gene ID #:
2140
NCBI Official Name:
eyes absent homolog 3 (Drosophila)
NCBI Official Symbol:
EYA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q99504
User NOte:
Optimal dilutions for each application to be determined by the researcher.