NCAM1 / CD56 Antibody

ProSci
Product Code: PSI-23-276
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-276-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NCAM1 / CD56 antibody (23-276) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using NCAM1 / CD56 antibody (23-276) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of C6 cells using NCAM1 / CD56 antibody (23-276) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NCAM1 / CD56 antibody (23-276) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using NCAM1 / CD56 antibody (23-276) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using NCAM1 / CD56 antibody (23-276) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
neural cell adhesion molecule, NCAM, antigen recognized by monoclonal 5.1H11, NCAM-1, N-CAM-1, MSK39, CD56, neural cell adhesion molecule 1, NCAM1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
NCBI Gene ID #:
4684
NCBI Official Name:
neural cell adhesion molecule 1
NCBI Official Symbol:
NCAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Immunology, Neuroscience, Stem Cell
Swissprot #:
P13591
User NOte:
Optimal dilutions for each application to be determined by the researcher.