SORT1 Antibody

ProSci
Product Code: PSI-23-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-283-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using SORT1 antibody (23-283) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human kidney using SORT1 antibody (23-283) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse liver using SORT1 antibody (23-283) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SORT1 antibody (23-283) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using SORT1 antibody (23-283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SORT1 antibody (23-283) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Gp95, LDLCQ6, NT3, NTR3, sortilin, 100 kDa NT receptor, glycoprotein 95, neurotensin receptor 3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 520-760 of human SORT1 (NP_002950.3).
NCBI Gene ID #:
6272
NCBI Official Name:
sortilin 1
NCBI Official Symbol:
SORT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q99523
User NOte:
Optimal dilutions for each application to be determined by the researcher.