WNT9A Antibody

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ProSci
Product Code: PSI-23-293
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-293-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using WNT9A antibody (23-293) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using WNT9A antibody (23-293) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse lung, using WNT9A antibody (23-293) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using WNT9A antibody (23-293) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Protein Wnt-9a, Protein Wnt-14, WNT9A, WNT14
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
NCBI Gene ID #:
7483
NCBI Official Name:
Protein Wnt-9a
NCBI Official Symbol:
WNT9A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
O14904
User NOte:
Optimal dilutions for each application to be determined by the researcher.