ADAM12 Antibody

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ProSci
Product Code: PSI-23-294
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-294-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ADAM12 antibody (23-294) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of C6 cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ADAM12 antibody (23-294) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (23-294) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Adam12, OTTMUSP00000023508, Mltna, mKIAA4001, meltrin-alpha, meltrin alpha, KIAA4001, disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, a disintegrin and metalloproteinase domain 12 (meltrin alpha), a disintegrin and metalloprotease domain 12, a disintegrin and metallopeptidase domain 12 (meltrin alpha), MLTN, UNQ346/PRO545
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2).
NCBI Gene ID #:
8038
NCBI Official Name:
ADAM metallopeptidase domain 12
NCBI Official Symbol:
ADAM12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
O43184
User NOte:
Optimal dilutions for each application to be determined by the researcher.