IRS2 Antibody

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ProSci
Product Code: PSI-23-299
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-299-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IRS2 antibody (23-299) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human esophagus using IRS2 antibody (23-299) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using IRS2 antibody (23-299) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human esophagus using IRS2 antibody (23-299) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Insulin receptor substrate 2, IRS-2, IRS2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human IRS2 (NP_003740.2).
NCBI Gene ID #:
8660
NCBI Official Name:
Insulin receptor substrate 2
NCBI Official Symbol:
IRS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 170kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Immunology, Signal Transduction
Swissprot #:
Q9Y4H2
User NOte:
Optimal dilutions for each application to be determined by the researcher.