SOCS5 Antibody

ProSci
Product Code: PSI-23-305
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-305-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SOCS5 antibody (23-305) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human liver cancer using SOCS5 antibody (23-305) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using SOCS5 antibody (23-305) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SOCS5 antibody (23-305) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver cancer using SOCS5 antibody (23-305) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SOCS5 antibody (23-305) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SOCS5, suppressor of cytokine signaling 5, CIS6, CISH6, KIAA0671, cytokine-inducible SH2 protein 6, CISH5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOCS5 (NP_054730.1).
NCBI Gene ID #:
9655
NCBI Official Name:
suppressor of cytokine signaling 5
NCBI Official Symbol:
SOCS5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
O75159
User NOte:
Optimal dilutions for each application to be determined by the researcher.