ARHGEF9 Antibody

ProSci
Product Code: PSI-23-314
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-314-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (23-314) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of A549 cells using ARHGEF9 antibody (23-314). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (23-314) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using ARHGEF9 antibody (23-314). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ARHGEF9, ARHDH9, EIEE8, HPEM-2, KIAA0424, PEM-2 homolog, PEM2, COLLYBISTIN, HPEM-2 collybistin, PEM-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).
NCBI Gene ID #:
23229
NCBI Official Name:
Cdc42 guanine nucleotide exchange factor (GEF) 9
NCBI Official Symbol:
ARHGEF9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 61kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O43307
User NOte:
Optimal dilutions for each application to be determined by the researcher.