PIP4K2B Antibody

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ProSci
Product Code: PSI-23-354
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-354-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (23-354) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (23-354) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PIP4K2B antibody (23-354) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B, PIP5K2B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PIP4K2B (NP_003550.1).
NCBI Gene ID #:
8396
NCBI Official Name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
NCBI Official Symbol:
PIP4K2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P78356
User NOte:
Optimal dilutions for each application to be determined by the researcher.