STK4 Antibody

Contact us
ProSci
Product Code: PSI-23-365
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-365-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cells, using STK4 antibody (23-365) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
4 / 4
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody (23-365). Western blot was performed from the immunoprecipitate using STK4 antibody (23-365) at a dilition of 1:1000.
❮❯

Western blot analysis of extracts of various cells, using STK4 antibody (23-365) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody (23-365). Western blot was performed from the immunoprecipitate using STK4 antibody (23-365) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, Serine/threonine-protein kinase 4 37kDa subunit, MST1/N, Serine/threonine-protein kinase 4 18kDa subunit, MST1/C, STK4, KRS2, MST1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human STK4 (NP_006273.1).
NCBI Gene ID #:
6789
NCBI Official Name:
Serine/threonine-protein kinase 4
NCBI Official Symbol:
STK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cell Cycle, Signal Transduction
Swissprot #:
Q13043
User NOte:
Optimal dilutions for each application to be determined by the researcher.