HMGCL Antibody

ProSci
Product Code: PSI-23-367
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-367-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HMGCL antibody (23-367) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using HMGCL antibody (23-367) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using HMGCL antibody (23-367) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using HMGCL antibody (23-367) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using HMGCL antibody (23-367) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HMGCL antibody (23-367) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
HMGCL, HL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human HMGCL (NP_000182.2).
NCBI Gene ID #:
3155
NCBI Official Name:
3-hydroxymethyl-3-methylglutaryl-CoA lyase
NCBI Official Symbol:
HMGCL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P35914
User NOte:
Optimal dilutions for each application to be determined by the researcher.