MED24 Antibody

ProSci
Product Code: PSI-23-372
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-372-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MED24 antibody (23-372) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.

Western blot analysis of extracts of various cell lines, using MED24 antibody (23-372) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.

Further Information

Additional Names:
ARC10CRSP10CRSP4, DRIP10MED5, THRAP4, TRAP10mediator of RNA polymerase II transcription subunit 24, CRSP complex subunit 4, activator-recruited cofactor 100 kDa component, cofactor required for Sp1 transcriptional activation subunit 4, cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa, mediator of RNA polymerase II transcription, subunit 24 homolog, thyroid hormone receptor-associated protein 4, thyroid hormone receptor-associated protein complex 100 kDa component, vitamin D3 receptor-interacting protein complex 100 kDa component, vitamin D3 receptor-interacting protein complex component DRIP100
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MED24 (NP_055630.2).
NCBI Gene ID #:
9862
NCBI Official Name:
mediator complex subunit 24
NCBI Official Symbol:
MED24
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75448
User NOte:
Optimal dilutions for each application to be determined by the researcher.