HSD11B2 Antibody

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ProSci
Product Code: PSI-23-384
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-384-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (23-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (23-384) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (23-384) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (23-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (23-384) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (23-384) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3
Application Note:
WB: 1:200 - 1:1000

IHC: 1:50 - 1:100
Background:
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
NCBI Gene ID #:
3291
NCBI Official Name:
hydroxysteroid (11-beta) dehydrogenase 2
NCBI Official Symbol:
HSD11B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P80365
User NOte:
Optimal dilutions for each application to be determined by the researcher.