NEDD4L Antibody

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ProSci
Product Code: PSI-23-389
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-389-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NEDD4L antibody (23-389) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody (23-389) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using NEDD4L antibody (23-389) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using NEDD4L antibody (23-389) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody (23-389) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using NEDD4L antibody (23-389) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD42, Nedd4-2, NEDD4L, KIAA0439, NEDL3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
NCBI Gene ID #:
23327
NCBI Official Name:
E3 ubiquitin-protein ligase NEDD4-like
NCBI Official Symbol:
NEDD4L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 113kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q96PU5
User NOte:
Optimal dilutions for each application to be determined by the researcher.