ARSF Antibody

ProSci
Product Code: PSI-23-400
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-400-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARSF antibody (23-400) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using ARSF antibody (23-400) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ARSF antibody (23-400) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ARSF antibody (23-400) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Arylsulfatase F, ASF, 316-, ARSF
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ARSF (NP_004033.2).
NCBI Gene ID #:
416
NCBI Official Name:
Arylsulfatase F
NCBI Official Symbol:
ARSF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 66kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P54793
User NOte:
Optimal dilutions for each application to be determined by the researcher.