CETN3 Antibody

ProSci
Product Code: PSI-23-408
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-408-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CETN3 antibody (23-408) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CETN3 antibody (23-408) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody (23-408) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CDC31, CEN3, centrin-3, CDC31 yeast homolog, EF-hand superfamily member, centrin, EF-hand protein, 3 (CDC31 homolog, yeast)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CETN3 (NP_004356.2).
NCBI Gene ID #:
1070
NCBI Official Name:
centrin 3
NCBI Official Symbol:
CETN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
O15182
User NOte:
Optimal dilutions for each application to be determined by the researcher.