NDUFA2 Antibody

ProSci
Product Code: PSI-23-424
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-424-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDUFA2 antibody (23-424) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver cancer using NDUFA2 antibody (23-424) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using NDUFA2 antibody (23-424) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver cancer using NDUFA2 antibody (23-424) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
B8, CD14, CIB8, MC1DN13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa, NADH-ubiquinone oxidoreductase subunit CI-B8, complex I B8 subunit
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human NDUFA2 (NP_002479.1).
NCBI Gene ID #:
4695
NCBI Official Name:
NADH:ubiquinone oxidoreductase subunit A2
NCBI Official Symbol:
NDUFA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
O43678
User NOte:
Optimal dilutions for each application to be determined by the researcher.