OXCT1 Antibody, KO Validated

Contact us
ProSci
Product Code: PSI-23-426
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-426-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using OXCT1 antibody (23-426) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 4
3 / 4
Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (23-426) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (23-426) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using OXCT1 antibody (23-426) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (23-426) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (23-426) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
OXCT1, OXCT, SCOT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
NCBI Gene ID #:
5019
NCBI Official Name:
3-oxoacid CoA transferase 1
NCBI Official Symbol:
OXCT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P55809
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.