POR Antibody, KO Validated

ProSci
Product Code: PSI-23-429
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-429-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using POR antibody (23-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 6
Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (23-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
3 / 6
Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (23-429) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 6
Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using POR antibody (23-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (23-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (23-429) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
POR, CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
NCBI Gene ID #:
5447
NCBI Official Name:
P450 (cytochrome) oxidoreductase
NCBI Official Symbol:
POR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 77kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P16435
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.