BCKDK Antibody

ProSci
Product Code: PSI-23-450
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-450-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BCKDK antibody (23-450) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using BCKDK antibody (23-450) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using BCKDK antibody (23-450) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using BCKDK antibody (23-450) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BCKDK antibody (23-450) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using BCKDK antibody (23-450) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using BCKDK antibody (23-450) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BCKDK antibody (23-450) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BCKDK, BDK, BCKDKD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).
NCBI Gene ID #:
10295
NCBI Official Name:
branched chain ketoacid dehydrogenase kinase
NCBI Official Symbol:
BCKDK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O14874
User NOte:
Optimal dilutions for each application to be determined by the researcher.