ARFGAP3 Antibody

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ProSci
Product Code: PSI-23-461
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-461-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (23-461) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver cancer using ARFGAP3 antibody (23-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using ARFGAP3 antibody (23-461) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (23-461) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver cancer using ARFGAP3 antibody (23-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using ARFGAP3 antibody (23-461) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
ARFGAP3, ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3).
NCBI Gene ID #:
26286
NCBI Official Name:
ADP-ribosylation factor GTPase activating protein 3
NCBI Official Symbol:
ARFGAP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9NP61
User NOte:
Optimal dilutions for each application to be determined by the researcher.