LLGL2 Antibody

ProSci
Product Code: PSI-23-488
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-488-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Immunofluorescence analysis of U2OS cells using LLGL2 antibody (23-488) at dilution of 1:100. Blue: DAPI for nuclear staining.
2 / 4
Immunohistochemistry of paraffin-embedded human prostate using LLGL2 antibody (23-488) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat lung using LLGL2 antibody (23-488) at dilution of 1:200 (40x lens).
4 / 4
Western blot analysis of extracts of various cell lines, using LLGL2 antibody (23-488) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.

Immunofluorescence analysis of U2OS cells using LLGL2 antibody (23-488) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human prostate using LLGL2 antibody (23-488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using LLGL2 antibody (23-488) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using LLGL2 antibody (23-488) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.

Further Information

Additional Names:
9130006H11Rik, Llglh2, lethal(2) giant larvae protein homolog 2, lethal giant larvae-like protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 620-819 of mouse LLGL2 (NP_663413.2).
NCBI Gene ID #:
217325
NCBI Official Name:
LLGL2 scribble cell polarity complex component
NCBI Official Symbol:
LLGL2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 113kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
User NOte:
Optimal dilutions for each application to be determined by the researcher.