DDX39B Antibody

ProSci
Product Code: PSI-23-529
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-529-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX39B antibody (23-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat ovary using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterus using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DDX39B antibody (23-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat ovary using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DDX39B antibody (23-529) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DDX39B Polyclonal Antibody (23-529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BAT1, BAT1, UAP56, D6S81E
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 319-428 of human DDX39B (NP_004631.1).
NCBI Gene ID #:
7919
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
NCBI Official Symbol:
DDX39B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13838
User NOte:
Optimal dilutions for each application to be determined by the researcher.