SFI1 Antibody

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ProSci
Product Code: PSI-23-533
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-533-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of MCF-7 cells, using SFI1 antibody (23-533) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Western blot analysis of extracts of MCF-7 cells, using SFI1 antibody (23-533) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Documents

Further Information

Additional Names:
PISD, PPP1R139, hSfi1p, protein SFI1 homolog, Sfi1 homolog, spindle assembly associated, homolog of yeast Sfi1, protein phosphatase 1, regulatory subunit 139
Application Note:
WB: 1:500 - 1:2000
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2).
NCBI Gene ID #:
9814
NCBI Official Name:
SFI1 centrin binding protein
NCBI Official Symbol:
SFI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 148kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
A8K8P3
User NOte:
Optimal dilutions for each application to be determined by the researcher.