METTL3 Antibody

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ProSci
Product Code: PSI-23-540
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-540-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using METTL3 antibody (23-540) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of HeLa cells using METTL3 antibody (23-540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody (23-540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody (23-540). Western blot was performed from the immunoprecipitate using METTL3 antibody (23-540) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using METTL3 antibody (23-540) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using METTL3 antibody (23-540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody (23-540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody (23-540). Western blot was performed from the immunoprecipitate using METTL3 antibody (23-540) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
METTL3, M6A, IME4, Spo8, MT-A70
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).
NCBI Gene ID #:
56339
NCBI Official Name:
methyltransferase like 3
NCBI Official Symbol:
METTL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q86U44
User NOte:
Optimal dilutions for each application to be determined by the researcher.