HLA-DMB Antibody

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ProSci
Product Code: PSI-23-547
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-547-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded human stomach using HLA-DMB antibody (23-547) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of Raji cells, using HLA-DMB antibody (23-547) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using HLA-DMB antibody (23-547) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of Raji cells, using HLA-DMB antibody (23-547) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein, HLA-DMB, DMB, RING7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2).
NCBI Gene ID #:
3109
NCBI Official Name:
HLA class II histocompatibility antigen, DM beta chain
NCBI Official Symbol:
HLA-DMB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
P28068
User NOte:
Optimal dilutions for each application to be determined by the researcher.