YTHDF3 Antibody

ProSci
Product Code: PSI-23-552
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-552-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using YTHDF3 Antibody (23-552) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using YTHDF3 antibody (23-552) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using YTHDF3 antibody (23-552) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using YTHDF3 antibody (23-552) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using YTHDF3 Antibody (23-552) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using YTHDF3 antibody (23-552) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using YTHDF3 antibody (23-552) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using YTHDF3 antibody (23-552) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
YTHDF3, FLJ31657,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human YTHDF3 (NP_689971.4).
NCBI Gene ID #:
253943
NCBI Official Name:
YTH domain family, member 3
NCBI Official Symbol:
YTHDF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q7Z739
User NOte:
Optimal dilutions for each application to be determined by the researcher.