ProSci

HK3 Antibody

Product Code:
 
PSI-23-576
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HK3 antibody (23-576) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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3 / 3
Immunohistochemistry of paraffin-embedded rat kidney using HK3 antibody (23-576) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using HK3 antibody (23-576) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using HK3 antibody (23-576) at dilution of 1:100 (40x lens).

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PSI-23-576-50uL50uL£433.00
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Further Information

Additional Names:
HK3, HKIII, Hexokinase 3, Hexokinase type III, HXK3, Hexokinase 3 (white cell), Hexokinase-3, HK III
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2).
NCBI Gene ID #:
3101
NCBI Official Name:
hexokinase 3 (white cell)
NCBI Official Symbol:
HK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P52790
User NOte:
Optimal dilutions for each application to be determined by the researcher.