PCK2 Antibody

ProSci
Product Code: PSI-23-590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-590-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PCK2 antibody (23-590) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 10s.
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Western blot analysis of extracts of various cell lines, using PCK2 antibody (23-590) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 10s.

Further Information

Additional Names:
PCK2, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK-M, PEPCK2, PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IP: 1:20 - 1:100
Background:
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).
NCBI Gene ID #:
5106
NCBI Official Name:
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
NCBI Official Symbol:
PCK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Cancer, Obesity, Signal Transduction
Swissprot #:
Q16822
User NOte:
Optimal dilutions for each application to be determined by the researcher.