PDC Antibody

ProSci
Product Code: PSI-23-591
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-591-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded mouse retina using PDC antibody (23-591) at dilution of 1:200 (40x lens).
2 / 3
Immunohistochemistry of paraffin-embedded rat retina using PDC antibody (23-591) at dilution of 1:200 (40x lens).
3 / 3
Western blot analysis of extracts of various cell lines, using PDC antibody (23-591) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Immunohistochemistry of paraffin-embedded mouse retina using PDC antibody (23-591) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat retina using PDC antibody (23-591) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using PDC antibody (23-591) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
Phosducin, PHD, 33 kDa phototransducing protein, Protein MEKA, PDC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a phosphoprotein, which is located in the outer and inner segments of the rod cells in the retina. This protein may participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. It modulates the phototransduction cascade by interacting with the beta and gamma subunits of the retinal G-protein transducin. This gene is a potential candidate gene for retinitis pigmentosa and Usher syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PDC (NP_002588.3).
NCBI Gene ID #:
5132
NCBI Official Name:
Phosducin
NCBI Official Symbol:
PDC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P20941
User NOte:
Optimal dilutions for each application to be determined by the researcher.