PER1 Antibody

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ProSci
Product Code: PSI-23-592
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-592-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PER1 antibody (23-592) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using PER1 antibody (23-592) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using PER1 antibody (23-592) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using PER1 antibody (23-592) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PER1 antibody (23-592) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PER1 antibody (23-592) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PER1 antibody (23-592) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PER1 antibody (23-592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Period circadian protein homolog 1, hPER1, Circadian clock protein PERIOD 1, Circadian pacemaker protein Rigui, PER1, KIAA0482, PER, RIGUI
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
NCBI Gene ID #:
5187
NCBI Official Name:
Period circadian protein homolog 1
NCBI Official Symbol:
PER1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Transcription
Swissprot #:
O15534
User NOte:
Optimal dilutions for each application to be determined by the researcher.