CHCHD3 Antibody

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ProSci
Product Code: PSI-23-673
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-673-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CHCHD3 antibody (23-673) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 40s.
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Immunohistochemistry of paraffin-embedded rat brain using CHCHD3 antibody (23-673) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using CHCHD3 antibody (23-673) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using CHCHD3 antibody (23-673) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 40s.
Immunohistochemistry of paraffin-embedded rat brain using CHCHD3 antibody (23-673) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CHCHD3 antibody (23-673) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
CHCHD3, FLJ20420, coiled-coil-helix-coiled-coil-helix domain containing 3, MINOS3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).
NCBI Gene ID #:
54927
NCBI Official Name:
coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol:
CHCHD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9NX63
User NOte:
Optimal dilutions for each application to be determined by the researcher.