EIF3J Antibody

ProSci
Product Code: PSI-23-694
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-694-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF3J antibody (23-694) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EIF3J antibody (23-694) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EIF3S1, eIF3-alpha, eIF3-p35, eukaryotic translation initiation factor 3 subunit J, eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
NCBI Gene ID #:
8669
NCBI Official Name:
eukaryotic translation initiation factor 3 subunit J
NCBI Official Symbol:
EIF3J
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75822
User NOte:
Optimal dilutions for each application to be determined by the researcher.