CACYBP Antibody

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ProSci
Product Code: PSI-23-718
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-718-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CACYBP antibody (23-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunofluorescence analysis of NIH-3T3 cells using CACYBP antibody (23-718) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of HeLa cells using CACYBP antibody (23-718) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CACYBP antibody (23-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of NIH-3T3 cells using CACYBP antibody (23-718) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using CACYBP antibody (23-718) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CACYBP, GIG5, MGC87971, PNAS-107, RP1-102G20.6, S100A6BP, SIP
Application Note:
WB: 1:1000 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1).
NCBI Gene ID #:
27101
NCBI Official Name:
calcyclin binding protein
NCBI Official Symbol:
CACYBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q9HB71
User NOte:
Optimal dilutions for each application to be determined by the researcher.