F2RL1 Antibody

ProSci
Product Code: PSI-23-725
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-725-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using F2RL1 antibody (23-725) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Western blot analysis of extracts of various cell lines, using F2RL1 antibody (23-725) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Further Information

Additional Names:
GPR11, PAR2, proteinase-activated receptor 2, G-protein coupled receptor 11, coagulation factor II (thrombin) receptor-like 1, protease-activated receptor 2, thrombin receptor-like 1
Application Note:
WB: 1:200 - 1:1000
Background:
Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 318-397 of human F2RL1 (NP_005233.3).
NCBI Gene ID #:
2150
NCBI Official Name:
F2R like trypsin receptor 1
NCBI Official Symbol:
F2RL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P55085
User NOte:
Optimal dilutions for each application to be determined by the researcher.