SS18L1 Antibody

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ProSci
Product Code: PSI-23-735
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-735-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescence analysis of HeLa cells using SS18L1 antibody (23-735) at dilution of 1:100. Blue: DAPI for nuclear staining.
2 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using SS18L1 antibody (23-735) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse brain using SS18L1 antibody (23-735) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using SS18L1 antibody (23-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of HeLa cells using SS18L1 antibody (23-735) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using SS18L1 antibody (23-735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SS18L1 antibody (23-735) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SS18L1 antibody (23-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
Calcium-responsive transactivator, SS18-like protein 1, SYT homolog 1, SS18L1, CREST, KIAA0693
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 90-270 of human SS18L1 (NP_945173.1).
NCBI Gene ID #:
26039
NCBI Official Name:
Calcium-responsive transactivator
NCBI Official Symbol:
SS18L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
O75177
User NOte:
Optimal dilutions for each application to be determined by the researcher.