VAMP1 Antibody

ProSci
Product Code: PSI-23-750
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-750-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using VAMP1 antibody (23-750) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat spleen using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using VAMP1 antibody (23-750) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat spleen using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using VAMP1 antibody (23-750) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SYB1, VAMP-1, SYB1, Synaptobrevin-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-96 of human VAMP1 (NP_954740.1).
NCBI Gene ID #:
6843
NCBI Official Name:
vesicle-associated membrane protein 1 (synaptobrevin 1)
NCBI Official Symbol:
VAMP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P23763
User NOte:
Optimal dilutions for each application to be determined by the researcher.