ATP5L Antibody

ProSci
Product Code: PSI-23-807
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-807-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP5L antibody (23-807) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using ATP5L antibody (23-807) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.

Further Information

Additional Names:
ATP5JG, ATP5L, ATP synthase subunit g, mitochondrial, ATP synthase g chain, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G, ATP synthase, H+ transporting, mitochondrial F1Fsubunit g, ATP synthase, H+ transporting, mitochondrial Fo complex subunit G, ATPase subunit G, F1F0-type ATP synthase subunit g, F1Fo-ATP synthase complex Fo membrane domain g subunit
Application Note:
WB: 1:500 - 1:2000
Background:
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).
NCBI Gene ID #:
10632
NCBI Official Name:
ATP synthase membrane subunit g
NCBI Official Symbol:
ATP5L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O75964
User NOte:
Optimal dilutions for each application to be determined by the researcher.