CALB2 Antibody

ProSci
Product Code: PSI-23-912
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-912-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CALB2 antibody (23-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded mouse lung using CALB2 antibody (23-912) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of rat brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CALB2 antibody (23-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse lung using CALB2 antibody (23-912) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CALB2, 29 kDa calbindin, CAL2, Calretinin, CAB29, Calbindin 2, Calbindin D29K, CR
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human CALB2 (NP_001731.2).
NCBI Gene ID #:
794
NCBI Official Name:
calbindin 2
NCBI Official Symbol:
CALB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P22676
User NOte:
Optimal dilutions for each application to be determined by the researcher.