BAZ1B Antibody, KO Validated

ProSci
Product Code: PSI-23-942
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-942-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BAZ1B antibody (23-942) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (23-942) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BAZ1B antibody (23-942) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (23-942) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Tyrosine-protein kinase BAZ1B, Bromodomain adjacent to zinc finger domain protein 1B, Williams syndrome transcription factor, Williams-Beuren syndrome chromosomal region 10 protein, Williams-Beuren syndrome chromosomal region 9 protein, hWALp2, BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
NCBI Gene ID #:
9031
NCBI Official Name:
Tyrosine-protein kinase BAZ1B
NCBI Official Symbol:
BAZ1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 190kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UIG0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.