BAF60a Antibody

ProSci
Product Code: PSI-23-994
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-994-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (23-994) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (23-994) (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (23-994) (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (23-994) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (23-994) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (23-994) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (23-994) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (23-994) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (23-994) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (23-994) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, BAF60A, Protein D15KZ1, SWI/SNF complex 60 kDa subunit, Smarcd1, Baf60a, D15Kz1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of mouse BAF60a (NP_114030.2).
NCBI Gene ID #:
83797
NCBI Official Name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1
NCBI Official Symbol:
SMARCD1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 58kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q96GM5
User NOte:
Optimal dilutions for each application to be determined by the researcher.