ADAM10 Antibody

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ProSci
Product Code: PSI-24-024
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-024-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ADAM10 antibody (24-024) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit<br />Exposure time: 30s.
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Immunofluorescence analysis of HeLa cells using ADAM10 antibody (24-024) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ADAM10 antibody (24-024) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit<br />Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using ADAM10 antibody (24-024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ADAM10 Antibody: RAK, kuz, AD10, AD18, MADM, CD156c, HsT18717, KUZ, Disintegrin and metalloproteinase domain-containing protein 10, CDw156, ADAM 10
Application Note:
WB 1:500 - 1:2000

IF 1:50 - 1:200
Background:
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 214-500 of human ADAM10 (NP_001101.1).
NCBI Gene ID #:
102
NCBI Official Name:
ADAM metallopeptidase domain 10
NCBI Official Symbol:
ADAM10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa, 105 kDa
Purification:
Affinity purification
Research Area:
Apoptosis,Cancer
Swissprot #:
O14672
User NOte:
Optimal dilutions for each application to be determined by the researcher.