IRAK4 Antibody

ProSci
Product Code: PSI-24-025
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-025-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using IRAK4 antibody (24-025) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit.<br />Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat testis using IRAK4 antibody (A6208) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse brain using IRAK4 antibody (A6208) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using IRAK4 antibody (24-025) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit.<br />Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using IRAK4 antibody (A6208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IRAK4 antibody (A6208) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64
Application Note:
WB 1:500 - 1:2000

IHC 1:50 - 1:200
Background:
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
NCBI Gene ID #:
51135
NCBI Official Name:
interleukin 1 receptor associated kinase 4
NCBI Official Symbol:
IRAK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55 kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9NWZ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.