ATG5 Antibody [1358CT289.125.123]

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ProSci
Product Code: PSI-64-229
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-64-229-100ul100ul£650.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 1358CT289.125.123
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from rat C6,Hela,mouse NIH/3T3,SH-SY5Y,U-87 MG cell line (from left to right), using ATG5 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Western blot analysis of lysates from rat C6,Hela,mouse NIH/3T3,SH-SY5Y,U-87 MG cell line (from left to right), using ATG5 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.

Documents

Further Information

Additional Names:
ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
Application Note:
For WB starting dilution is: 1:1000
Background:
Involved in autophagy vesicles formation. Conjugation with ATG12 through an ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. HCV utilizes ATG5 as a proviral factor during the onset of viral infection. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures; as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
Background References:
  • Hammond E.M.,et al.FEBS Lett. 425:391-395(1998).
  • Chen Y.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
  • Bechtel S.,et al.BMC Genomics 8:399-399(2007).
  • Mungall A.J.,et al.Nature 425:805-811(2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Bovine
Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human APG5L(82-97aa DRFDQFWAINRKLMEY), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
NCBI Gene ID #:
9474
NCBI Official Name:
Autophagy protein 5
NCBI Official Symbol:
ATG5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Human=32,23 kDa
Mouse=32 kDa
Rat=25 kDa
Protein Accession #:
Q9H1Y0
Purification:
Immunogen affinity purified.
Research Area:
Autophagy
Swissprot #:
Q9H1Y0
User NOte:
Optimal dilutions for each application to be determined by the researcher.