AKT1 (phospho S473) Antibody [H4]

ProSci
Product Code: PSI-54-321
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-54-321-100ug100ug£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Recombinant Monoclonal Rabbit IgG / light chain kappa
Antibody Clonality: Monoclonal
Antibody Clone: H4
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at 4°C for up to 6 months. Store at -20°C long term

Images

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Antibody: 54-321 at 1/1000 dilution<br>Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane<br>Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/60 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
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Antibody: 54-321 at 1/1000 dilution<br>Sample: Lane 1: 1ug of PDGF treated NIH-3T3 cell lysate . Lane 2: 500ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads<br>Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/66 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%

Antibody: 54-321 at 1/1000 dilution<br>Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane<br>Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/60 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
Antibody: 54-321 at 1/1000 dilution<br>Sample: Lane 1: 1ug of PDGF treated NIH-3T3 cell lysate . Lane 2: 500ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads<br>Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/66 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%

Further Information

Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Application Note:
WB, IP dilution (1:1000 ? 1: 5000).
Buffer:
49% PBS, 50% Glycerol, 0.05% BSA, pH 7.2, 0.02% NaN3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Also recommended for detection of phosphor-AKT1 in sheep & zebrafish
Immunogen:
Synthetic peptides derived from human AKT1 (RPHFPQFsYSASGTA)
Modifications:
Phospho-Specific
NCBI Gene ID #:
207
NCBI Official Name:
AKT serine/threonine kinase 1
NCBI Official Symbol:
AKT1
NCBI Organism:
homo sapiens
Purification:
Protein A purified
Research Area:
Signal Transduction, Cancer, Obesity
Swissprot #:
P31749
User NOte:
Optimal dilutions for each application to be determined by the researcher.