BACE1 Antibody [3C1C3]

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ProSci
Product Code: PSI-32-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-238-0.1mL0.1mL£626.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3C1C3
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
BACE monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
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Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
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Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Documents

Further Information

Additional Names:
BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2
Application Note:
ELISA: 1/10000
Western Blot: 1/500 - 1/2000
ICC: 1/200 - 1/1000
Flow Cytometry: 1/200 - 1/400
Background:
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene.
Background References:
  • J Neurochem. 2012 Jan;120 Suppl 1:62-70.
  • Eur J Neurosci. 2010 Oct;32(7):1223-38.
Buffer:
Purified antibody in PBS with 0.05% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli.
NCBI Gene ID #:
23621
NCBI Official Name:
beta-secretase 1
NCBI Official Symbol:
BACE1
NCBI Organism:
Homo sapiens
Research Area:
Neuroscience
Swissprot #:
P56817
User NOte:
Optimal dilutions for each application to be determined by the researcher.