TIGIT Antibody [2C7]

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ProSci
Product Code: PSI-SD8829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-SD8829-0.02mg0.02mg£166.00
Quantity:
PSI-SD8829-0.1mg0.1mg£531.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Llama
Antibody Isotype: sdAb
Antibody Clonality: Monoclonal
Antibody Clone: 2C7
Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
TIGIT antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.
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Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.
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Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.
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Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
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Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.
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Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.
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Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.
Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.
Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.
Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.
Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.

Documents

Further Information

Additional Names:
TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Application Note:
TIGIT antibody can be used for ELISA starting at 0.1 μg/mLimmunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 10μg/mL.
Background:
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Background References:
  • Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
  • Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.
Buffer:
TIGIT Antibody is supplied in PBS.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
NCBI Gene ID #:
2016332
NCBI Official Name:
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol:
TIGIT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_776160
Protein GI Number:
256600228
Purification:
TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.??It also contains a myc-tag for detection.
Research Area:
Immunology
Swissprot #:
Q495A1
User NOte:
Optimal dilutions for each application to be determined by the researcher.