LAG-3 Antibody [2B11]
Code | Size | Price |
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PSI-SD8841-0.02mg | 0.02mg | £166.00 |
Quantity:
PSI-SD8841-0.1mg | 0.1mg | £531.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Llama
Antibody Isotype: sdAb
Antibody Clonality: Monoclonal
Antibody Clone: 2B11
Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
LAG3 antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
Application Note:
LAG-3 antibody can be used for ELISA starting at 0.1 μg/mL. For immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL. For flow cytometry at 1 μg/ml.
Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Background References:
- Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216?21.
- Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619?22.
- Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885?91.
- Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970?9.
Buffer:
LAG-3 Antibody is supplied in PBS.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
NCBI Gene ID #:
3902
NCBI Official Name:
lymphocyte activating 3
NCBI Official Symbol:
LAG3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002277
Protein GI Number:
167614500
Purification:
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.??It also contains a myc-tag for detection.
Research Area:
Immunology
Swissprot #:
P18627
User NOte:
Optimal dilutions for each application to be determined by the researcher.